The Dac-tag, an affinity tag based on penicillin-binding protein 5 |
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Authors: | Lee David Wei Peggie Mark Deak Maria Toth Rachel Gage Zoe Olivia Wood Nicola Schilde Christina Kurz Thimo Knebel Axel |
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Institution: | Advantagen, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK. |
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Abstract: | Penicillin-binding protein 5 (PBP5), a product of the Escherichia coli gene dacA, possesses some β-lactamase activity. On binding to penicillin or related antibiotics via an ester bond, it deacylates and destroys them functionally by opening the β-lactam ring. This process takes several minutes. We exploited this process and showed that a fragment of PBP5 can be used as a reversible and monomeric affinity tag. At ambient temperature (e.g., 22°C), a PBP5 fragment binds rapidly and specifically to ampicillin Sepharose. Release can be facilitated either by eluting with 10mM ampicillin or in a ligand-free manner by incubation in the cold (1-10°C) in the presence of 5% glycerol. The "Dac-tag", named with reference to the gene dacA, allows the isolation of remarkably pure fusion protein from a wide variety of expression systems, including (in particular) eukaryotic expression systems. |
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