Characterization of <Emphasis Type="Italic">Brassica juncea</Emphasis> antioxidant potential under salinity stress |
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Authors: | Pankaj Kumar Joshi Saurabh Chandra Saxena Sandeep Arora |
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Institution: | (1) Department of Biochemistry, College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology, U. S. Nagar, Pantnagar, Uttarakhand, 263145, India;(2) Department of Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology, U. S. Nagar, Pantnagar, Uttarakhand, 263145, India; |
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Abstract: | Present study characterizes the anti-oxidative defense potential of four Brassica juncea varieties, Pusa Jaikisan, Varuna, RLM-198, and CS-52, differing in their ability to withstand salinity stress. 7-day-old
seedlings raised in MS medium supplemented with 0, 50, 100, and 150 mM NaCl were used to monitor changes in the growth profile,
level of stress marker molecules, and activities of important antioxidant enzymes. Increasing NaCl concentration resulted
in a significant (P ≤ 0.05) reduction of shoot fresh and dry mass and vigor index in all the varieties tested. Maximum reduction in growth was
recorded for RLM-198 while CS-52 maintained better growth characteristics. Varuna and RLM-198 exhibited a limited increase
in superoxide dismutase, ascorbate peroxidase, and total peroxidase activity under increasing salinity. These varieties also
recorded maximum salt stress-induced damage in terms of increased lipid peroxidation, H2O2 content, and electrolyte leakage. On the other hand, CS-52 recorded maximum proline accumulation with minimum levels of H2O2, electrolyte leakage, and malondialdehyde contents. With increasing salinity stress, CS-52 recorded maximal increase in the
activity of antioxidant enzymes. However, catalase activity did not correlate with alterations in H2O2 levels under stress. Interestingly, a lower superoxide dismutase:ascorbate peroxidase ratio in CS-52 correlated with stress
tolerance trait, while a comparatively higher superoxide dismutase:ascorbate peroxidase ratio in RLM-198 marked the susceptible
nature of the variety. Our results propose that superoxide dismutase:ascorbate peroxidase ratio is the critical factor, determining
the degree of stress tolerance in Brassica juncea. |
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