Application of hydrogenase in biotechnological conversions |
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| Authors: | A van Berkel-Arts M Dekker C van Dijk H J Grande W R Hagen R Hilhorst M Krüse-Wolters C Laane C Veeger |
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| Affiliation: | 1. University of Chinese Academy of Sciences, Beijing 100049, China;2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;3. National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China |
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| Abstract: | Evidence will be presented in this review article that the application of hydrogenase has large biotechnological possibilities. Our investigations show: Fast reaction of hydrogenase at an electrode surface to reduce H+; Photochemical production of H2 by hydrogenase by photosensitized Ru-complexes dissolved in reversed micellar membranes and vectorial H+ transport through the membrane to the water phase; The production of fine chemicals in reversed micelles by a system containing specific enzymes, hydrogenase and H2. The rules to obtain maximal conversion rates with this system will be presented. |
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