| 利用mini-Tn10转座系统研究芽孢杆菌生物被膜形成相关基因 |
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| 引用本文： | 高卫华,郝建安,夏思源,徐海津,白艳玲,张秀明,乔明强.利用mini-Tn10转座系统研究芽孢杆菌生物被膜形成相关基因[J].微生物学通报,2009,36(3):0345-0349. |
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| 作者姓名： | 高卫华 郝建安 夏思源 徐海津 白艳玲 张秀明 乔明强 |
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| 作者单位： | 1. 南开大学生命科学学院,天津,300071;分子微生物学与技术教育部重点实验室,天津,300071
2. 南开大学生命科学学院,天津,300071 |
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| 基金项目： | 天津市应用基础研究计划重点项目(No. 033605011) |
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| 摘 要： | 生物被膜对于细菌抵御外界环境的侵害具有重要的意义, 其形成和发展过程受到很多基因的调控和影响。本文利用mini-Tn10转座系统对野生型解淀粉芽孢杆菌NK10.BAhjaWT进行突变库的构建并随机选择400个转化子进行验证, 突变效率达到90%以上。从突变库中筛选到4株生物被膜缺陷株。经过鉴定, 上述突变株citB、citG、gpsA和yvfB基因发生插入突变。其中citB、citG和gpsA均与能量代谢相关, yvfB功能未知。本实验证明mini-Tn10转座系统对于芽孢杆菌突变库的构建具有高效和稳定的
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| 关 键 词： | 生物被膜 解淀粉芽孢杆菌 mini-Tn10转座系统 |
Using Mini-Tn10 Transposon System to Research the Genes Involved in Biofilm Formation in Bacillus |
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| GAO Wei-Hu,HAO Jian-An,XIA Si-Yuan,XU Hai-Jin,BAI Yan-Ling,ZHANG Xiu-Ming and QIAO Ming-Qiang.Using Mini-Tn10 Transposon System to Research the Genes Involved in Biofilm Formation in Bacillus[J].Microbiology,2009,36(3):0345-0349. |
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| Authors: | GAO Wei-Hu HAO Jian-An XIA Si-Yuan XU Hai-Jin BAI Yan-Ling ZHANG Xiu-Ming and QIAO Ming-Qiang |
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| Institution: | (1. Institute for Molecular Biology, College of Life Science, NanKai University, Tianjin 300071, China)(2. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin 300071, China);Institute for Molecular Biology, College of Life Science, NanKai University, Tianjin 300071, China;Institute for Molecular Biology, College of Life Science, NanKai University, Tianjin 300071, China;Institute for Molecular Biology, College of Life Science, NanKai University, Tianjin 300071, China;Institute for Molecular Biology, College of Life Science, NanKai University, Tianjin 300071, China;Institute for Molecular Biology, College of Life Science, NanKai University, Tianjin 300071, China;(1. Institute for Molecular Biology, College of Life Science, NanKai University, Tianjin 300071, China)(2. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin 300071, China) |
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| Abstract: | Biofilm has great influence for bacteria to resist the harm from outer environment. Its forming and development are controlled by many different genes. In this research, an insertional mutagenesis library of a wide type Bacillus amyloliquefaciens NK10.BAhjaWT was built via mini-Tn10 transposon system to find out genes involved in biofilm formation. Four such genes have been screened, in which, citB, citG, gpsA are all related to the energy metabolism. The function of another gene of yvfB is unknown. In a word, mini-Tn10 transposon system was proved to be efficient and stable in Bacillus. |
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| Keywords: | Biofilm Bacillus amyloliquefaciens Mini-Tn10 transposon system |
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