Properties of ribosomal binding sites for phenylalanyl-transfer ribonucleic acid |
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| Authors: | N W Seeds J A Retsema T W Conway |
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| Affiliation: | 1. Administration Department, Indiana Aerospace University, IAU Town Center, Kagudoy Road, Lapu-Lapu City, Cebu, Philippines;2. Teacher Education Department, Indiana Aerospace University, IAU Town Center, Kagudoy Road, Lapu-Lapu City, Cebu, Philippines;3. Hospitality and Tourism Management Department, Indiana Aerospace University, IAU Town Center, Kagudoy Road, Lapu-Lapu City, Cebu, Philippines;4. General Education Department, Indiana Aerospace University, IAU Town Center, Kagudoy Road, Lapu-Lapu City, Cebu, Philippines;5. Airline Management Department, Indiana Aerospace University, IAU Town Center, Kagudoy Road, Lapu-Lapu City, Cebu, Philippines;6. Pure Sciences Department, College of Arts and Sciences, Cebu Technological University, M.J. Cuenco Avenue Corner R. Palma Street, Cebu City, 6000, Cebu, Philippines;1. Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt;2. Department of Dermatology, STD’s and Andrology, Faculty of Medicine, Minia University, Al Minya, Egypt;1. Dipartimento di Farmacia, Università “G. D’Annunzio” Chieti-Pescara, Via dei Vestini 31, 66013 Chieti Scalo, CH, Italy;2. Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland;3. Institut für Pharmazie der Universität Innsbruck, Abteilung Pharmakognosie, Innrain 80/82, A-6020 Innsbruck, Austria;4. Dipartimento di Scienze Agrarie ed Ambientali, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy |
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| Abstract: | [14C]Phenylalanyl-tRNA bound to Escherichia coli ribosomes in the presence of poly U and varying levels of deacylated tRNA was isolated by adsorption to cellulose nitrate filters. The extent of binding of the radioisotope was inhibited by deacylated tRNA in a manner suggesting two binding sites for phenylalanyl-tRNA one sensitive to inhibition by deacylated tRNA and the other insensitive. The data did not fit a model that assumed identical sites with equal affinity for deacylated or acylated tRNA. Combinations of either tetracycline or streptomycin and deacylated tRNA gave additive inhibitions, while a mixture of both antibiotics did not produce a greater effect than either tested alone. Thus the antibiotics may inhibit the binding of phenylalanyl-tRNA at one type of ribosomal site, whereas deacylated tRNA may inhibit binding at the other. |
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