Plant regeneration from protoplasts of long-term callus cultures of Actinidia deliciosa var. deliciosa cv. Hayword (Kiwifruit) |
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Authors: | M Margarida Oliveira M Salomé S Pais |
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Institution: | (1) Departamento de Biologia Vegetal, Faculdade de Ciências de Lisboa, Bloco C2, 1700 Campo Grande, Lisboa, Portugal |
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Abstract: | Plant regeneration of Actinidia deliciosa var. deliciosa cv. Hayword was obtained from protoplasts isolated from petiole derived long-term callus cultures. Protoplasts were cultured in liquid medium over agarose gelled medium. Regenerated green callus, plated on solid medium, could develop shoots that rooted spontaneously in hormone-less medium. The plants obtained are growing fast in soil and present a normal phenotype.Abbreviations BAP
benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- DTT
dithiotreitol
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- Kin
kinetin
- MES
2-(N-morpholino) ethanesulphonic acid
- MS
Murashige and Skoog (1962) medium
- NAA
naphthalene-1-acetic acid
- SH
Schenk and Hildebrandt (1972) medium
This Research was supported by JNICT and INIC |
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Keywords: | |
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