大白鼠中缝核一氧化氮合酶阳性神经元的组织化学观察

中脑和脑桥部中缝核被认为与睡眠有直接和间接关系的重要脑结构。本文用一氧化氮合酶（ＮＯＳ）组织化学结合荧光组织化学方法证实在中缝核群中，ＮＯＳ阳性神经元主要定位于这两个脑部的中缝核内，ＮＯＳ产生的ＮＯ能使脑血管扩张，参与脑血流的调节。提示这二个脑部中缝核内的ＮＯＳ阳性神经元可能作为多种因素之一，参于睡眠状态下基本脑血流的维持

HISTOCHEMICAL STUDY ON NITRIC OXIDE SYNTHASE POSITIVE NEURONS IN THE RAPHE NUCLEI OF THE RAT

The distribution of nitric oxide synthase (NOS) positive neurons in the raphe nuclei of the rat was studied by the nicotinamide adenine dinucleotid diaphorase (NADPH d) histochemistry combinedon with histofluorescence method. The results showed that NOS positive neurons were mainly located in serotoninergic raphe nuclei of the midbrain and pons including the caudal linear, dorsal, madian, and pontine raphe nucleus. These raphe nuclei have benn considered to be directly responsible for slow wave sleep (SWS) and indirectly for paradoxical sleep ( P S). NO has been reported to dilate cerebral vessels. Thus the NOS positive neurons residing in the raphe nucleus suggest that NO generated by NOS positive neurons may be involved in the maintenance of basal cerbral flow during sleep.