Immunoaffinity purification and quantitation of a rat protein similar to the human decidua-associated protein (hDP) 71 |
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| Authors: | R. Halperin E. Hadas N. Shinnar P.F. Kraicer I. Bukovsky D. Schneider |
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| Affiliation: | 1. Department of Obstetrics and Gynecology, Assaf Harofe Medical Center, Zerifin, Affiliated to the Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel;2. Peptor Laboratories, Science Based Industrial Park, Israel;3. Department of Zoology, George S, Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel;1. Division of Restorative and Prosthetic Dentistry, The Ohio State University College of Dentistry, 305 West 12th Avenue, Room 3005H, Columbus, OH 43210-1267, USA;2. Oral Biology, The Ohio State University College of Dentistry, 305 West 12th Avenue, Room 3037D, Columbus, OH 43210-1267, USA;1. National Institute of Technology, Warangal, India;2. Department of Computer Science and Engineering, National Institute of Technology, Warangal, India;1. Izmir Katip Celebi University, Faculty of Engineering, Department of Engineering Sciences, Çiğli, Izmir, Turkey;2. Toros University, Faculty of Engineering, Electric & Electronic Department, Mersin, Turkey;3. Izmir Institute of Technology, Material Science and Engineering, Izmir, Turkey;4. Izmir Katip Celebi University, Faculty of Engineering, Department of Material Science and Engineering, Çiğli, Izmir, Turkey;5. Ege University, Solar Energy Institute, Izmir, Turkey |
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| Abstract: | The role of human decidua-associated protein (hDP) 71, copurified consistently with hDP 200, identified as rheumatoid factor, remains undetermined. The possibility of using a rat as an experimental model for the further research of hDp 71, was examined. A rat protein, similar to the hDp 71, was immunoaffinity purified using the same monoclonal antibody recognizing hDp 71. The protein was named rat decidua- associated protein (rDP) 71. The level of hDp 71 in extracts of endometrial epithelium and stroma, as well as in uterine washings, was measured throughout the oestrous cycle and on 5 consecutive days, starting the day after the rats mated. Moreover, the effect of oestrogen and progesterone on the level of hDp 71 was examined. The results demonstrate the oestrogen-dependent accumulation of rDP 71 in uterine lumen, and support the use of a rat as an experimental animal model to investigate the possible physiological role of this protein in the reproductive process. |
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| Keywords: | rat decidua-associated protein 71 rheumatoid factor human decidua-associated protein (hDp) 200 estrogen effect progesterone |
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