A Simple,Large-Scale Overexpression Method of Deriving Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium Carboxydothermus hydrogenoformans |
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| Abstract: | We established an Na2S-free, large-scale overexpression system of deriving CODH II from thermophilic bacterium Carboxydothermus hydrogenoformans in Escherichia coli using a large-scale fermentor. Recombinant-CODH II showed a CO oxidation activity of 9,600 U/mg. In addition, recombinant-CODH II exhibited considerable CO2 reduction activity, of 16.9 U/mg. |
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| Keywords: | overexpression metalloenzyme CO dehydrogenase CO2 reduction |
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