Simple Method for Analyzing Phosphoamino Acids by Thin-layer Chromatography: Application to Elongation Factor 1 from Wheat Embryo

A simple and rapid method was developed separating phosphoserine, phosphothreonine, phosphotyrosine, phosphoric acid, and ATP by one-dimensional cellulose thin-layer chromatography, using a freshly prepared solvent system of 1-butanol–formic acid–water (5:2:1). This system was applied to determine phosphoamino acid (phosphoserine) of wheat elongation factor 1β phosphorylated by casein kinase II from the wheat embryo.