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A Simple and Efficient Method for High Fidelity PCR Cloning Using Antibody-neutralizing Technology
Abstract:We introduce the TA cloning antibody method for the high-fidelity PCR product amplified by family B DNA polymerase without purification. This method uses antibodies and Thermus aquaticus (Taq) DNA polymerase. The antibodies can inhibit only the activity of family B DNA polymerase, and Taq can co-work for A-tailing. This method has nearly cloning efficiency to that of the PCR product of Taq.
Keywords:high-fidelity PCR product  family B DNA polymerase  KOD DNA polymerase  TA cloning method  antibody
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