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Mutational Analysis of the Multicopy hao Gene Coding for Hydroxylamine Oxidoreductase in Nitrosomonas sp. Strain ENI-11 |
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| Abstract: | The ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11 contains three copies of the hao gene (hao 1, hao 2, and hao 3) coding for hydroxylamine oxidoreductase (HAO). Three single mutants (hao 1::kan, hao 2::kan, or hao 3::kan) had 68 to 75% of the wild-type growth rate and 58 to 89% of the wild-type HAO activity when grown under the same conditions. A double mutant (hao 1::kan and hao 3::amp) also had 68% of the wild-type growth and 37% of the wild-type HAO activity. |
| Keywords: | ammonia-oxidizing bacterium gene disruption hydroxylamine oxidoreductase multicopy gene Nitrosomonas |