Enzymatic Peptide Synthesis with Fatty Acid-modified Chymotrypsin and Trypsin in Aqueous-organic Media

Equilibrium controlled enzymatic peptide syntheses in aqueous-organic media were done with chymotrypsin and trypsin modified with varied fatty acid. Palmitoyl-modified chymotrypsin and trypsin synthesized the peptide at 1.7 and 2.4 times higher yield than those unmodified. Since an alteration of the substrate specificity was not observed, the chemical modification did not affect the active site of the enzymes.