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Efficient Expression of a Transfected Foreign Gene by Cos1 Cells in Serum-free Medium
Abstract:In this report, we have found that fatty acid-free bovine serum albumin stimulated the expression of a transfected foreign gene in Cos1 cells in serum-free medium and that its activity was as same as that of fetal calf serum. This will simplify the purification of the gene product from the culture medium.
Keywords:lactosucrose  β-fructofuranosidase  transfructosylation  kinetic analysis  oligosaccharide
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