Identification of the structural gene for yeast cytochrome c oxidase subunit I on mitochondrial DNA. |
| |
Authors: | E Keyhani |
| |
Institution: | Laboratory for Cell Biology and Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 314-1700, Tehran, Iran |
| |
Abstract: | Mitochondrial protein synthesis was analyzed in the yeast mit? mutants of which specifically lack cytochrome oxidase. 3H]leucine labeled polypeptides synthesized in yeast OXI 3 mutant were analyzed by means of immunoprecipitation and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). When compared to control, subunit I was not detectable. This result was substantiated by growing OXI 3 mutant in the presence of cycloheximide, an inhibitor of cytoplasmic protein synthesis. Under such conditions SDS-PAGE analysis of 3H]leucine labeled immunoprecipitate shows the absence of subunit I. These data show that the OXI 3 locus contains the structural gene for cytochrome oxidase subunit I. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|