Chemoinformatics-assisted development of new anti-biofilm compounds |
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Authors: | Anna Dürig Irene Kouskoumvekaki Rebecca M Vejborg Per Klemm |
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Institution: | (1) Department of Biochemistry and Microbiology, Rutgers, The State University of New Jersey, 76 Lipman Drive, New Brunswick, NJ, USA;(2) Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research, Leipzig, Germany; |
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Abstract: | The fuel oxygenate, methyl tert-butyl ether (MTBE), although now widely banned or substituted, remains a persistent groundwater contaminant. Multidimensional
compound-specific isotope analysis (CSIA) of carbon and hydrogen is being developed for determining the extent of MTBE loss
due to biodegradation and can also potentially distinguish between different biodegradation pathways. Carbon and hydrogen
isotopic fractionation factors were determined for MTBE degradation in aerobic and anaerobic laboratory cultures. The carbon
isotopic enrichment factor (εC) for aerobic MTBE degradation by a bacterial consortium containing the aerobic MTBE-degrading bacterium, Variovorax paradoxus, was −1.1 ± 0.2‰ and the hydrogen isotope enrichment factor (εH) was −15 ± 2‰. This corresponds to an approximated lambda value (Λ = εH/εC) of 14. Carbon isotope enrichment factors for anaerobic MTBE-degrading enrichment cultures were −7.0 ± 0.2‰ and did not vary
based on the original inoculum source, redox condition of the enrichment, or supplementation with syringic acid as a co-substrate.
The hydrogen enrichment factors of cultures without syringic acid were insignificant, however a strong hydrogen enrichment
factor of −41 ± 3‰ was observed for cultures which were fed syringic acid during MTBE degradation. The Λ = 6 obtained for NYsyr cultures might be diagnostic for the stimulation of anaerobic MTBE degradation by methoxylated compounds
by an as yet unknown pathway and mechanism. The stable-isotope enrichment factors determined in this study will enhance the
use of CSIA for monitoring anaerobic and aerobic MTBE biodegradation in situ. |
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