Chromosomal location of the Escherichia coli cytochrome b556 gene, cybA |
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Authors: | Hiroshi Murakami Kiyoshi Kita Hiroshi Oya Yasuhiro Anraku |
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Affiliation: | (1) Department of Biology, Faculty of Science, University of Tokyo, 113 Hongo, Tokyo, Japan;(2) Department of Parasitology, Juntendo University, School of Medicine, 113 Hongo, Tokyo, Japan |
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Abstract: | Summary The amounts of cytochrome b556 in the cytoplasmic membranes of several Escherichia coli K12 strains having F-prime factors and a lambda transducing phage were determined. The amount was amplified about two-fold in strains having F100-12 and F152, but not in strains having F100-11, F8 and psu+2glnS+. The strain TK3D11, which lacks the kdp-gltA region (deletion D-01) of the E. coli chromosome, did not synthesize cytochrome b556 at all. From these results, the gene cybA encoding cytochrome b556 was located in the kdp-gltA region.In the cytochrome b556-deficient mutant, a novel b type cytochrome, cytochrome b561 which is a product of the gene cybB, was identified. It seems to function as a physiological electron transferring cytochrome in place of cytochrome b556 in this mutant.Abbeviations HPLC high performance liquid chromatography - EDTA ethylenediamine tetraacetic acid - SDS sodium dodecyl sulfate - NADH reduced form of nicotinamide adenine dinucleotide |
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