Poly(ADP-ribose) effectively competes with DNA for histone H4 binding |
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| Authors: | G Sauermann J Wesierska-Gadek |
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| Affiliation: | 1. Division of Nephrology, West China Hospital of Sichuan University, Chengdu, Sichuan, China;2. Division of Nephrology, Mianyang Central Hospital, Mianyang, China;3. Division of Pathology, West China Hospital of Sichuan University, Chengdu, Sichuan, China. |
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| Abstract: | The effect of poly(ADP-ribose) on DNA-histone H4 interaction was studied using a nitrocellulose filter binding assay. Poly-(ADP-ribose) was found to form poly(ADP-ribose)-histone H4 complexes at physiological salt concentrations. The homopolymer effectively competed with DNA for histone H4 binding. Poly(ADP-ribose) was also capable of displacing DNA from preformed DNA-histone H4 complexes. Our hypothesis is that poly(ADP-ribose), locally and transiently formed at the site of DNA damage, causes dissociation of DNA from the nucleosome particle or nucleosome unfolding. |
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