In vivo differentiation of adipose-derived stem cells in an injectable poloxamer-octapeptide hybrid hydrogel |
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| Authors: | Huang Jinlong Wang Shukui Wei Cui Xu Yan Wang Ying Jin Jiyang Teng Gaojun |
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| Institution: | aPlastic Reconstructive Surgery & Medical Aesthetic Center, Nanjing University of TCM Affiliated Hospital, Nanjing, PR China;bDepartment of Radioloy, Zhongda Hospital of Southeast University, Nanjing, PR China;cCentral Lab of Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, PR China;dNanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing, PR China |
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| Abstract: | A hybrid hydrogel (PP) composed of Polomaxer-407 (PO) and octapeptide with amino acid sequence of KFEFKFEF (PE) was prepared to make a scaffold material incorporating PO's high and tunable mechanical strength and integrity with PE's superior bioactivity. Human adipose-derived mesenchymal stem cells (hASCs) were encapsulated into PE, PO and PP hydrogels respectively and injected subcutaneously at the dorsal neck area of nude mice. Adipose-like tissue regeneration was only observed in the mice injected with cell-encapsulated PP hydrogel. No adipose regeneration was found in the mice injected with PO or PE. Immunohistochemistry analysis with mouse anti-human nuclei monoclonal antibody demonstrated that the cells in the regenerated adipose-like tissue was originated from the injected hASCs. The growth of blood capillaries indicated that the regenerated adipose-like tissue was living tissue. In addition, human-originated cells were also found in nude mice skin. These cells were positive with mouse anti-human cells keratin antibody, suggesting that the injected hASCs migrated to the skin and differentiated into epithelial cells in vivo. |
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| Keywords: | Hydrogel Scaffold Soft tissue defects Adipose tissue engineering Adipose-derived stem cells |
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