Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay |
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Authors: | Jiang Zhong-Liang Li Qing-Wang Li Wen-Ye Hu Jian-Hong Zhao Hong-Wei Zhang Shu-Shan |
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Institution: | College of Animal Science & Technology, Northwest A & F University, Yangling, ShaanXi Province, PR China. |
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Abstract: | A modified protocol of neutral comet assay was utilized to assess the effect of low density lipoprotein (LDL) on the DNA integrity of boar freezing-thawing semen. The results demonstrated that the method was high sensitive and easier manipulation and LDL significantly protected sperm DNA integrity (p<0.05) from the damage caused by cryopreservation except TD at the concentration of 6%, 7% and TM at 6%, the optimal LDL concentration in diluents was 9%. Moreover, LDL showed better protection in 0.25 ml than in 0.5 ml types of straw (p<0.05) and no difference was observed in the same volume straw at the concentration of 9% and 10%. It was just the same for LDL effect on boar sperm DNA in cryopreservation 0 day and 30 days (p>0.05). |
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