Immunocytochemical Detection of Anti-Hippocampal Antibodies in Alzheimer's Disease and Normal Cerebrospinal Fluid |
| |
Authors: | Loeffler David A. Juneau Paul L. Nguyen Hanh-Uyen Najman Dina Pomara Nunzio LeWitt Peter A. |
| |
Affiliation: | (1) Neuroscience Program, Sinai Hospital, Detroit, Michigan, 48235;(2) Division of Geriatric Psychiatry, Nathan S. Kline Institute for Psychiatric Research, Orangeburg, New York, 10962;(3) Departments of Neurology and Psychiatry, Wayne State University School of Medicine, Detroit, Michigan, 48201 |
| |
Abstract: | Immunocytochemical staining was performed to investigate the presence of anti-hippocampal antibodies in cerebrospinal fluid (CSF) from patients with probable Alzheimer's disease (AD) (n = 19), aged normal controls (n = 9), and young normal controls (n = 10). Marked staining of neurons in the granule cell layer of the dentate gyrus and in pyramidal neurons in CA1-3 of the rat hippocampus was observed in 5 AD CSF samples (26%), 1 aged control sample (11%), and 1 young control sample (10%). These differences were not statistically significant. One of the immunoreactive AD CSF specimens also contained high concentrations of C5b-9, the membrane attack complex. The infrequent occurrence of anti-hippocampal antibodies in AD CSF, and the detection of similar immunoreactivity in control CSF specimens, suggest that these antibodies are unlikely to play a role in the neurodegenerative process in most individuals with AD. However, elevated C5b-9 concentration in an AD CSF specimen with marked immunoreactivity to hippocampal neurons suggests the possibility that anti-neuronal antibodies may contribute to complement activation in some AD patients. |
| |
Keywords: | Alzheimer's disease antibodies cerebrospinal fluid complement hippocampus |
本文献已被 SpringerLink 等数据库收录! |
|