Assay of L-aromatic amino acid decarboxylase by high performance liquid chromatography |
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| Authors: | M D'Erme M A Rosei A Fiori G Di Stazio |
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| Affiliation: | 1. Istituto di Chimica Biologica, Facoltà di Farmacia, Università di Roma, Centro di Biologia Molecolare del C.N.R., Roma, Italy;2. Laboratori Ricerche Polifarma, Via Tor Sapienza 138, Roma, Italy |
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| Abstract: | A method is presented for the assay, by high-performance liquid chromatography, of l-aromatic amino acid decarboxylase. The Km value of the enzyme for 3,4-dihydroxyphenylalanine (dopa) was, by this procedure, 0.16 mm, in good agreement with previous reports. When α-methyldopa was used as substrate, evidence was obtained indicating the formation of, besides α-methyldopamine, 3,4-dihydroxyphenylacetone, presumably through an internal transamination process. |
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| Keywords: | Current address as stated for reprint requests. |
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