Immunoassay method to check the flagellin mediated binding of stenotrophomonas maltophilia to polystyrene

Plate count and spectrophotometric methods have been used to asses the ability of an organism to attach to different surfaces and form biofilms. In the present study we report a highly sensitive, specific and quick method to check the role of flagellin in bacterial adhesion to polystyrene. Flagellin from Stenotrophomonas maltophilia showed high affinity for polystyrene (P < 0.05), which decreased on pretreatment offlagellin with anti-flagellin in a dilution dependent manner. In an enzyme immunoassay format a positive correlation was detected between the anti-flagellin dilutions and flagellin attachment to polystyrene (correlation coefficient +0.860155). These evidences conclusively prove the involvement of flagella in the adhesion ofS. maltophilia to polystyrene surface and enzyme immunoassay, a quick and reliable method to check this phenomenon.