Use of large particles support for fast analysis of methadone and its primary metabolite in human plasma by liquid chromatography-mass spectrometry |
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Authors: | Souverain S Rudaz S Ortelli D Varesio E Veuthey J L |
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Institution: | Laboratory of Analytical Pharmaceutical Chemistry, University of Geneva, 20 Boulevard d'Yvoy, 1211 4, Geneva, Switzerland. |
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Abstract: | A bioanalytical method was developed for the quantitation of methadone (MTD) and its primary metabolite, (EDDP) in plasma. The extraction step was performed within a capillary column packed with large particles (35x0.3 mm I.D.; d(p) 30 micrometer) at high flow-rate conditions (450 microliter/min). The separation was performed on a microbore analytical column (55x2 mm I.D.; d(p) 3 micrometer) coupled to a mass spectrometer (MS). This procedure was based on a column-switching unit. Analytes of interest were retained on the precolumn by hydrophobic interactions and backflushed from the precolumn to the analytical column. The detection was carried out with a MS single quadrupole equipped with an electrospray interface. The total analysis time was 6 min. The limits of quantification were evaluated at 10 and 25 ng/ml for MTD and EDDP, respectively. At this level, good accuracies were obtained for both analytes with repeatability values less than 18%. |
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Keywords: | Column switching Large particle support Fast analysis Methadone 2-Ethylidene-1 5-dimethyl-1 3-diphenylpyrrolidine |
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