Activation of diacylglycerol O-acyltransferase 1 gene results in increased tumor necrosis factor-alpha gene expression in 3T3-L1 adipocytes |
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| Authors: | Hirata Takashi Unoki Hiroyuki Bujo Hideaki Ueno Koichi Saito Yasushi |
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| Affiliation: | Department of Geriatric Pharmacology and Therapeutics, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan. |
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| Abstract: | The tumor necrosis factor-alpha (TNF-alpha) expression has been reported to be largely dependent on the size of adipocytes. We herein investigated the gene regulation of diacylglycerol O-acyltransferase (DGAT) in order to clarify the mechanism of TNF-alpha expression induced in large adipocytes. 3T3-L1 cells were cultured in the presence of 5 mM or 25 mM glucose to generate adipocytes from which the triglyceride content differs. The expression of TNF-alpha, DGAT1, and DGAT2 were upregulated in adipocytes cultured with 25 mM glucose. Furthermore, knockdown of DGAT1 gene significantly inhibited the TNF-alpha expression. Finally, the DGAT1 expression levels were closely related to the TNF-alpha level in 3T3-L1 adipocytes. |
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| Keywords: | TNF-α, tumor necrosis factor-α TG, triglyceride DGAT, diacylglycerol O-acyltransferase DMEM, Dulbecco’s modified Eagle’s medium Adipocytes with 5 mM glucose, adipocytes differentiated with constant 5 mM glucose condition Adipocytes with 25 mM glucose, adipocytes differentiated with constant 25 mM glucose conditions siRNA(s), small interfering RNA(s) |
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