葫芦苏铁遗传多样性的等位酶分析

采用等位酶技术,分析了葫芦苏铁5个居群的遗传多样性。分析6个酶系统,共获得了13个基因位点, 结果表明:葫芦苏铁具有较高水平的遗传变异性,多态位点百分率(P)为56．9％,等位基因平均数A=1.62,等 位基因多样性指数Ho=0.105,He=0．164,居群杂合体过量的位点为50％。居群遗传结构分析表明,大部分 遗传变异(93．6％)存在于居群内(FST=0．064),居群间分化程度甚微。根据葫芦苏铁的居群遗传结构式样并 结合相关研究,提出了就地保护所有居群,迁地保护从东一、王下和水田三个大居群取样的保护策略。

Genetic diversity of Cycas changjiangensis detected by allozyme analysis

Genetic diversity of 5 natrual populations of Cycaschangjiangensis were investigatied by using alloz-yme analysis. Allozyme data for 13 loci of 6 enzymes demonstrate high levels genetic variation within populations,the mean percentage of polymorphic loci(P)was 56. 9%. The mean number of alleles per locus(A)was 1. 62. Mean observed heterozygosity(Ho)was 0. 105,and mean expected heterozygosity(He)was 0. 164. Half of the Wright's F-statistics showed negative values. The analysis on population genetic structure indicated that most variation(93. 6%)resided within populations, with FST = 0. 064,thus mean the genetic differentiation between populations was low. On the basis of this study and other studies have been achived.we put forward the in-situ conservation scheme of all populations,and ex-situ conservation of sampling from the Dongyi.Wan-gxia and Shuitian populations of Cycas changjiangensis.