Cultivation of Saccharomyces cerevisiae in continuous culture |
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Authors: | Hans Georg W. Leuenberger |
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Affiliation: | (1) Abteilung für Biophysikalische Strahlenforschung, Gesellschaft für Strahlen- und Umweltforschung, Frankfurt a.M. |
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Abstract: | Summary Glucose limited growth of a respiratory deficient mutant of Saccharomyces cerevisiae was studied in continuous culture under steady state conditions. The maximal growth rate, the Michaelis constant, the cell yield, the maintenance coefficient and the ethanol yield of the growing cell population were determined. The steady state concentrations of cells, glucose and ethanol were measured as functions of the dilution rate and compared with theoretical predictions. A far-reaching agreement between theory and experiment was observed. The decrease of the cell yield in the range of low dilution rates is well explained by introducing the concept of maintenance energy in the general theory of continuous cultures. A deviation of the cell yield from the predicted values, which has been found in the range of high dilution rates, is discussed. |
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