Chemical quantitation of hemoglobin glycosylation: fluorometric detection of formaldehyde released upon periodate oxidation of glycoglobin |
| |
| Authors: | P M Gallop R Flückiger A Hanneken M M Mininsohn K H Gabbay |
| |
| Institution: | Children''s Hospital Medical Center and the Departments of Biological Chemistry, Oral Biology, Pediatrics, The Harvard Schools of Medicine and Dental Medicine, Boston, Massachusetts 02115, USA |
| |
| Abstract: | A sensitive fluorometric method for the quantitation of hemoglobin glycosylation, based upon periodate oxidation of the carbohydrate moieties present on both the α- and ?-amino groups of globin is described. The formaldehyde product is measured as the fluorescent 3,5-diacetyl-1,4-dihydrolutidine formed from the condensation of formaldehyde with acetylacetone and ammonia.This method is rigorously designed to assay glycosylated hemoglobin levels and to give a direct measure of the number of glycogroups per milligram of hemoglobin. It requires only 1 mg of protein and may also be used to determine the extent of the nonenzmatic glycosylation of other proteins. |
| |
| Keywords: | To whom correspondence should be addressed: Children's Hospital Medical Center 300 Longwood Avenue Boston Massachusetts 02115 |
| 本文献已被 ScienceDirect 等数据库收录！ |
