A rapid method for preparation of the cerebrospinal fluid proteome |
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| Authors: | Eivind Larssen Cato Brede Anne Bjørnstad Hjelle Kjell Birger Øysæd Anne Bolette Tjensvoll Roald Omdal Peter Ruoff |
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| Affiliation: | 1. Research Department, Stavanger University Hospital, Stavanger, Norway;2. International Research Institute of Stavanger, IRIS Envrionment, Stavanger, Norway;3. Department of Medical Biochemistry, Stavanger University Hospital, Stavanger, Norway;4. Department of Neurology, Stavanger University Hospital, Stavanger, Norway;5. Clinical Immunology Unit, Department of Internal Medicine, Stavanger University Hospital, Stavanger, Norway;6. Department of Medical Science, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway;7. Center for Organelle Research (CORE), University of Stavanger, Stavanger, Norway |
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| Abstract: | The cerebrospinal fluid (CSF) proteome is of great interest for investigation of diseases and conditions involving the CNS. However, the presence of high‐abundance proteins (HAPs) can interfere with the detection of low‐abundance proteins, potentially hindering the discovery of new biomarkers. Therefore, an assessment of the CSF subproteome composition requires depletion strategies. Existing methods are time consuming, often involving multistep protocols. Here, we present a rapid, accurate, and reproducible method for preparing the CSF proteome, which allows the identification of a high number of proteins. This method involves acetonitrile (ACN) precipitation for depleting HAPs, followed by immediate trypsination. As an example, we demonstrate that this method allows discrimination between multiple sclerosis patients and healthy subjects. |
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| Keywords: | ACN‐precipitation Cerebrospinal fluid LC‐MS/MS Sample preparation Technology Trypsination |
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