A study of free radicals in paraffin embedded and deparaffinized human heart muscle tissue using electron spin resonance (ESR) |
| |
Authors: | G. Elek A. Rockenbauer É. Magyar L. Jókay |
| |
Affiliation: | (1) Department of Pathology, Railway Hospital, Budapest, Hungary;(2) Central Research Institute for Chemistry of the Hungarian Academy of Sciences, P.O. Box 17, H-1525 Budapest, Hungary;(3) Department of Pathological Anatomy of Postgraduate Medical School, Budapest, Hungary |
| |
Abstract: | Summary Free radicals (spectroscopic splitting factor; g factor=2.003–2.005) were investigated in formol-fixed, paraffin embedded heart-muscle tissue sections using electron spin resonance (ESR) spectra. Changes in signal amplitude, g factor and line width were registered during deparaffinization, chloroform-methanol extraction, vapour treatment and bromination. An attempt was made to identify the source of the ESR signals by a correlation between the signal amplitude and number of fluorescent and/or Sudan-black-positive granules counted in the tissue sections. An increase in signal amplitude, g value and line narrowing were characteristic of the ascorbyl radical after deparafinization in air. Vapour treatment revelated that the broader signal has lower g factor, a characteristic that is tentatively assigned to oxidized lipids. The bromination resistant minor fraction of free radical centres with small g factor might be associated with the pigment content of the samples. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|