Gonadal steroid-dependent neuronal circuitries in avian limbic and preoptic regions.

In the avian limbic and preoptic region, the sexually dimorphic medial preoptic nucleus and nucleus of the stria terminalis are characterized by the presence of a testosterone-dependent aromatase-immunoreactive neuronal population. In situ hybridization studies confirmed that testosterone is modulating the expression of aromatase gene. Both nuclei are also characterized by a sexually dimorphic, testosterone-dependent vasotocin system. Immunocytochemical and in situ hybridization data, demonstrated that dimorphism and testosterone-sensitivity of this system are both dependent by an embryonic organizational effect of the estradiol. Intracerebroventricularly injected vasotocin, has a profound inhibitory effect on the male sexual behaviour, and immunocytochemical investigations revealed close associations among vasotocin fibres and aromatase cell bodies. These data suggest that this neuropeptidergic system could have a key role in the circuitries controlling different aspects of male reproductive behaviour in the Japanese quail.