Distribution of Na+, K+-ATPase α-Subunit Isoforms in Rat Pituitary |
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| Authors: | Paul M Rowe William T Link Charlene P Osborn Harold Gainer R Wayne Albers |
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| Institution: | Laboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892. |
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| Abstract: | The distributions of alpha-subunit isoforms of the Na+,K(+)-ATPase in rat pituitary were determined by immunoblotting and immunohistochemistry. Immunoreactivity for all three forms is present in the neural lobe, whereas the anterior lobe contains only alpha 1 and alpha 2. Most areas of the intermediate lobe exhibit faint immunoreactivity for only alpha 1, but thin strands of cells which stain strongly for all three isoforms are also present in this lobe. The previously reported ouabain inhibitable Na+,K(+)-ATPase activity in the neural lobe is consistent with the presence of both alpha 2 and alpha 3 subunits. |
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| Keywords: | Na+ K+-ATPase Isoforms Pituitary Immunoblotting Immunohistochemistry |
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