DNA methylation imprints on the IG-DMR of the Dlk1-Gtl2 domain in mouse male germline |
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| Authors: | Hiura Hitoshi Komiyama Junichi Shirai Motomu Obata Yayoi Ogawa Hidehiko Kono Tomohiro |
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| Affiliation: | Department of BioScience, Tokyo University of Agriculture, 1-1-1, Sakuragaoka, Tokyo 156-8502, Japan. |
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| Abstract: | Mouse genomes show a large cluster of imprinted genes at the Dlk1-Gtl2 domain in the distal region of chromosome 12. An intergenic-differentially methylated region (IG-DMR) located between Dlk1 and Gtl2 is specifically methylated in the male germline; IG-DMR regulates the parental allele-specific expression of imprinted genes. Here, we show the resetting of IG-DMR methylation marks during male germ-cell differentiation. For parental allele-specific methylation analysis, polymorphisms were detected in a 2.6-kb IG-DMR in three mouse strains. Bisulfite methylation analysis showed erasure of the marks by E14 and re-establishment before birth. The IG-DMR methylation status was maintained in spermatogonia and spermatocytes of mature testes. The IG-DMR methylation status established before birth is thus maintained throughout the lifetime in the male germline. |
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| Keywords: | IG-DMR, intergeneic-differentially methylated region PGCs, primordial germ cells BSA, bovine serum albumin GFP, green fluorescent protein DMEM, Dulbecco’s modified Eagle’s medium SNPs, single nucleotide polymorphisms |
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