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Spermidine levels and its relationship to DNA synthesis in outgrowing spores and vegetative cells of Bacillus subtilis
Authors:D Ginsberg  U Bachrach  A Keynan
Affiliation:1. Developmental Biology, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel;2. Department of Molecular Biology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel
Abstract:It is now established that the light-harvesting chlorophyll—protein complex (LHCP) of chloroplasts becomes phosphorylated in the light. In this study subfractionation of phosphorylated intact chloroplasts has been carried out to compare the phosphorylation of LHCP in non-appressed and appressed thylakoid regions. The results show around 10-times higher relative phosphorylation in the non-appressed regions than in the appressed ones. Since the non-appressed thylakoids also contain almost all photosystem 1, this region is likely to be the site for energy transfer from LHCP to photosystem 1 under phosphorylated conditions.
Keywords:Protein phosphorylation  Thylakoid organization  Chloroplast subfractionation  Light-harvesting chlorophyll—protein complex  Energy transfer  Membrane lateral heterogeneity  Chl  chlorophyll  LHCP  PS  photosystem  SDS  sodium dodecyl sulfate  PAGE  polyacrylamide gel electrophoresis
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