Cellobiose metabolism in Erwinia: genetic study |
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Authors: | Frederic Barras Jean Pierre Chambost Marc Chippaux |
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Affiliation: | (1) Laboratoire de Chimie Bactérienne, CNRS, 31, chemin Joseph Aiguier, F-13277 Marseille Cedex 9, France |
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Abstract: | Summary The study of mutants of Erwinia specifically unable to ferment cellobiose indicates that the mutations are clustered between arg and ile on the chromosome of this organism. In vivo cloning of the genes responsible for cellobiose utilization lead to a plasmid, pBEC2, which complements all Erwinia Clb- specific mutants. When introduced into wild-type E. coli it allows this organism to use cellobiose, arbutin and salicin; it also complements bglB and bglC mutants of Escherichia coli indicating that arbutin and salicin utilization is due to the products of the pBEC2 cloned genes. From the characterization of mutants pleiotropically affected in the utilization of various carbon sources, including cellobiose, arbutin and salicin, it is proposed that the three- -glucosides are substrates of the phosphoenolpyruvate-dependent phosphotransferase system (PTS). |
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