Chemiluminescence assay for tetrahydrobiopterin based on the generation of hydrogen peroxide using isoluminol-microperoxidase in the presence of 1-methoxy PMS. |
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| Authors: | Hidetoshi Arakawa Kouichi Masuda Noriko Tajima Masako Maeda |
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| Affiliation: | School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan. arakawa@pharm.showa-u.ac.jp |
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| Abstract: | We developed a novel highly sensitive chemiluminescence (CL) method for BH(4). The principle of the proposed method is based on active oxygen formation induced by 1-methoxy-5-methyl phenazinium methyl sulphate (1-methoxy PMS) in the presence of dissolved oxygen. Furthermore, active oxygen is determined by a CL assay involving the luminol reaction with microperoxidase. In this report, we examined the mechanism of formation and identified the reactive oxygen species derived from BH(4) employing 1-methoxy PMS. Additionally, optimum conditions for the CL assay of BH(4) were established. |
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| Keywords: | tetrahydrobiopterin chemiluminescence 1‐methoxy‐5‐methyl phenazinium methyl sulphate hydrogen peroxide luminol |
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