| 葡萄糖与胰岛素对3T3-F442A脂肪细胞中Leptin表达的调节 |
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| 引用本文： | 王方年,马春姑,张乃娴,宋后燕.葡萄糖与胰岛素对3T3-F442A脂肪细胞中Leptin表达的调节[J].生物化学与生物物理学报,1999(3). |
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| 作者姓名： | 王方年 马春姑 张乃娴 宋后燕 |
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| 作者单位： | 上海医科大学分子遗传学研究室 |
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| 摘 要： | 为了解葡萄糖与胰岛素对３Ｔ３Ｆ４４２Ａ脂肪细胞中Ｌｅｐｔｉｎ表达的调节，应用ＲＴＰＣＲ方法以ｂｅｔａａｃｔｉｎ为内对照对不同葡萄糖和／或胰岛素浓度培养条件下３Ｔ３Ｆ４４２Ａ脂肪细胞中ＬｅｐｔｉｎｍＲＮＡ表达水平进行相对定量分析。结果表明葡萄糖与胰岛素对３Ｔ３Ｆ４４２Ａ脂肪细胞中Ｌｅｐｔｉｎ表达有促进作用，过高浓度的葡萄糖抑制Ｌｅｐｔｉｎ的表达及胰岛素对Ｌｅｐｔｉｎ表达的促进。葡萄糖和胰岛素对Ｌｅｐｔｉｎ表达的促进作用无协同效应，且这种促进作用表现出饱和性特点。葡萄糖浓度的变化对脂肪细胞中Ｌｅｐｔｉｎ的表达与调控具有十分重要的影响。
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| 关 键 词： | 肥胖 脂肪细胞 胰岛素 葡萄糖 Leptin |
Glucose and Insulin Regulate Leptin Expression in 3T3 F442A Adipocytes |
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| WANG Fang
Nian,MA Chun Gu,ZHANG Nai Xia and SONG Hou Yan.Glucose and Insulin Regulate Leptin Expression in 3T3 F442A Adipocytes[J].Acta Biochimica et Biophysica Sinica,1999(3). |
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| Authors: | WANG Fang
Nian MA Chun Gu ZHANG Nai Xia and SONG Hou Yan |
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| Abstract: | Relative
quantitation RT PCR was used to investigate the regulation of leptin expression in 3T3 F442A
adipocytes by glucose and insulin. The results showed that glucose and insulin stimulated the
expression of leptin in 3T3 F442A adipocytes, but they did not act synergically. Over high
concentration of glucose suppressed the expression of leptin and the effect of insulin. The
elevation of the expression of leptin was characterized with saturation. The concentration of
glucose is very important for the regulation of leptin expression. |
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| Keywords: | Obesity adipocytes insulin glucose leptin |
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