Purification of S-2-hydroxyacylglutathione hydrolase (glyoxalase II) from rat erythrocytes. |
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| Authors: | J C Ball D L Vander Jagt |
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| Institution: | 1. Department of Biochemistry, University of New Mexico, Albuquerque, New Mexico 87131 USA;2. Department of Chemistry, University of New Mexico, Albuquerque, New Mexico 87131 USA |
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| Abstract: | Glyoxalase II, a specific glutathione thiolesterase, has been purified 9100-fold from rat erythrocytes using a purification scheme which employs Affi-Gel blue as a hydrophobic affinity column and also employs a glutathione-affinity column prepared by coupling S-(p-chlorophenacyl)glutathione to Affi-Gel 202. This procedure offers a convenient method for the preparation of highly purified glyoxalase II. Also described is a convenient method for the preparation of S-lactoyl-glutathione, a substrate for glyoxalase II. |
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| Keywords: | To whom correspondence should be sent |
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