A method for the selective isolation ofMyxococcus directly from soil |
| |
| Authors: | J P Karwowski G N Sunga S Kadam J B McAlpine |
| |
| Institution: | (1) Natural Product Discovery, Pharmaceutical Products Research and Development, Abbott Laboratories, 60064-3500 Abbott Park, Illinois, USA;(2) Dept 47P, Abbott Laboratories, Bldg AP9A, 100 Abbott Park Road, 60064-3500 Abbott Park, IL, USA |
| |
| Abstract: | A new method is described for the selective isolation of species ofMyxococcus directly from soil by dilution plating. The method involves suppression of competing microorganisms with antibiotics combined with air drying and wet heat treatment of soils. Fungi were eliminated by supplementing the plating medium with cycloheximide and nystatin. Non-sporulating bacteria were controlled by air drying soils and then heating aqueous soil dilutions for 10 min at 56°C. The predominant sporulating bacteria in soil,Streptomyces andBacillus, were suppressed by adding either tiacumicin B, ristocetin or vancomycin to the medium. Swarming ofMyxococcus colonies was controlled with a casein digest-yeast extract plating medium (CY-C10 agar). Ultrasound treatment of soil suspensions gave the highest number ofMyxococcus colonies in the soils studied, but these cultures could be recovered without ultrasound. Strains ofMyxococccus fulvus, M. xanthus, M. coralloides, M. stipitatus andM. virescens were isolated from soil using this technique. Soils examined yielded one or twoMyxococcus species per sample. |
| |
| Keywords: | myxobacteria Myxococcus soil selective isolation direct isolation method |
| 本文献已被 SpringerLink 等数据库收录！ |
|
