X射线辐射对仔鼠消化酶活性及胃中Bax和Ghrelin表达的影响

为了探讨X射线辐射对仔鼠胃蛋白酶活性、十二指肠脂肪酶活性及胃中Bax蛋白和Ghrelin表达的影响,对170只仔鼠用不同辐射剂量（0,4,12,20,28 Gy）X射线进行全身辐射,分别在辐射后1,5,10,20 d用比色法检测仔鼠胃蛋白酶活性和十二指肠中脂肪酶活性的变化,用免疫组织化学方法检测胃中Bax蛋白和Ghrelin的表达和分布,并用Image-proplus 5.0专业图像分析软件检测Bax蛋白和Ghrelin在胃中的表达强度。结果表明,X射线辐射影响发育期仔鼠胃蛋白酶和十二指肠脂肪酶的活性以及胃中Bax蛋白和Ghrelin的表达。仔鼠胃蛋白酶活性除在辐射后1 d时高于对照组外,其它辐射后各期均低于对照组,仔鼠十二指肠中脂肪酶活性在辐射后均低于对照组;Bax蛋白主要在仔鼠胃黏膜上皮细胞中表达,其表达水平随辐射剂量的增大而增强;Ghrelin主要在胃内分泌细胞中表达,辐射后其表达水平降低。X射线辐射影响仔鼠消化酶活性,这可能与胃中Bax蛋白和Ghrelin的表达变化有关。

Effects of X-ray on Activity of Digestive Enzyme and Expression of Bax and Ghrelin in Stomach of Fifal Mice

To explore the effects of X-ray on activity of pepsin,lipase of duodenum and expression of Bax and Ghrelin in stomach of fifal mice,170 mice were irradiated with different dosages（0,4,12,20,28 Gy） of X-ray.At 1～20 d after irradiation,detected activity of pepsin and lipase by colorimetry,studied distribution and positive expression of Bax and Ghrelin by immunohistochemical technology and analyzed them by IPP5.0.Results showed that X-ray radiation affects activities of pepsin,lipase and expression of Bax and Ghrelin in stomach of fifal mice.In irradiated group,the activities of pepsin are higher than control group on the first day after irradiated in stomach,and in other periods the activities are always lower than control group of fifal mice.In irradiated group,the activities of lipase of duodenum are all always lower than control group.Bax protein immunoreaction appeared in epithelial cells of stomach mucosa,the intensity increased with dosages augment.Ghrelin immunoreaction appeared in gland cells of stomach mucosa,the intensity reduced with dosages augment.X-ray radiation affects the activity of digestive enzyme of fifal mice,and this effect might be correlated with the change of positive expression of Bax protein and Ghrelin in stomach.