| C.elegans中dyn-1基因对寿命和发育的影响 |
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| 引用本文： | 曹蕾,杨玉荣. C.elegans中dyn-1基因对寿命和发育的影响[J]. 细胞生物学杂志, 2012, 0(8): 795-802 |
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| 作者姓名： | 曹蕾 杨玉荣 |
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| 作者单位： | 厦门大学生命科学学院,厦门361005 |
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| 基金项目： | 国家自然科学基金（No.30370695,No.30972181）资助项目 |
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| 摘 要： | 细胞极性对于细胞的多样性起着很重要的作用。发动蛋白是一个大的GTP酶,作用于胞吞作用和肌动蛋白的动力学过程。C.elegans中发动蛋白的同源基因dyn-1起着维持早期细胞极性的功能。我们对C.elegans中dyn-1基因进行了克隆,并构建到表达载体和RNAi载体中。经IPTG诱导表达得到了约90 kDa的DYN-1融合蛋白。同时,利用RNAi方法研究了dyn-1基因沉默后对三种线虫虫株N2、daf-2（e1370）和daf-16（e1038）寿命的影响。C.elegans在喂食dyn-1 RNAi食物后寿命明显缩短,也会导致严重的不育和胚胎致死。
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| 关 键 词： | C.elegans 细胞极性 发动蛋白 |
Effects of Caenorhabditis elegans dyn-1 on Lifespan and Fecundity |
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| Cao Lei,Yang Yurong. Effects of Caenorhabditis elegans dyn-1 on Lifespan and Fecundity[J]. Chinese Journal of Cell Biology, 2012, 0(8): 795-802 |
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| Authors: | Cao Lei Yang Yurong |
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| Affiliation: | (School of Life Science, Xiamen University, Xiamen 361005, China) |
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| Abstract: | Cell polarity is crucial for the generation of cell diversity. Dynamin, a large GTPase, functions in both endocytosis and actin dynamics. Here, the C. elegans dynamin ortholog, DYN-1, maintains anterior polarity cues. dyn-1 was amplified from C. elegans cDNA by PCR and cloned into an expression vector and a RNA interference vector. The effects ofdyn-1 on lifespan of N2, dqf-2（e1370） and daf-16（e1038） were studied by RNAi. The lifespans of N2, daf-2（e1370） and daf-16（e1038） were shortened significantly. In this study, we also observed sterility and embryonic lethality in the dyn-1 RNAi-treated worms. |
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| Keywords: | C. elegans cell polarity dynamin |
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