RCMV感染星形胶质细胞对神经干细胞分化的影响

探讨大鼠巨细胞病毒（rat cytomegalovirus，RCMV）感染大鼠星形胶质细胞后，对神经干细胞分化的影响。原代分离培养新生大鼠星形胶质细胞和胚胎海马神经干细胞，将星形胶质细胞感染RCMV后和神经干细胞在Transwell24孔共培养体系下进行共培养，同时设对照组；用免疫荧光染色等方法检测神经干细胞与感染RCMV的星形胶质细胞共培养后，其分化细胞中神经元微管相关蛋白（microtubule-associated protein 2，MAP2）和星形胶质细胞胶质纤维酸性蛋白（glial fibril—lary acidic protein，GFAP）的表达。结果发现，感染RCMV的星形胶质细胞与神经干细胞共培养时，神经干细胞分化减慢，分化成的神经元和星形胶质细胞比率低于对照组，提示星形胶质细胞感染RCMV后可抑制神经干细胞的分化，可能与RCMV影响星形胶质细胞合成和分泌各种营养因子，干扰了神经干细胞的分化进程有关。

The Effect of RCMV Infecting Astrocytes on Neural Stem Cells Differentiation

To investigate the effects of rat cytomegalovirus （RCMV） infected astrocytes on neural stem cells （NSCs） differentiation, the astrocytes and embryonic hippocampal neural stem cells were isolated and primary cultured from SD rats. NSCs were co-cultured with RCMV infected astrocytes in Transwell 24-well plate system with respective control groups. After NSCs were co-cultured with RCMV infected astrocytes, immunofluorescence staining was performed to study the expression of MAP2 and GFAP on neurons and astrocytes, respectively. The results showed that after co-culturing astrocytes with NSCs, the NSC differentiation slowed down and the differentiated neurons to astrocytes ratio was lower compared with the control group, suggesting that astrocytes infected with RCMV can inhibit the differentiation of NSCs. The possible mechanism may be related with the nutritional factors secreted from astrocytes, but affected by RCMV infection and thus interfered the differentiation process of NSCs.