| 蝴蝶兰合子和胚的分离 |
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| 引用本文： | 伍成厚,李冬妹,田惠桥.蝴蝶兰合子和胚的分离[J].细胞生物学杂志,2012(4):361-365. |
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| 作者姓名： | 伍成厚 李冬妹 田惠桥 |
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| 作者单位： | [1]厦门大学生命科学学院,厦门361005 [2]广州市园林科学研究所,广州510405 [3]顺德职业技术学院,顺德528300 |
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| 基金项目： | 广东省科技计划项目（No.20078020801007）和广州市科技计划项目（No.11A63030233）资助项目 |
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| 摘 要： | 该文用酶解一振荡法分离蝴蝶兰受精后的胚囊，然后显微解剖出合子、早期原胚及接近成熟的球形原胚。酶解液由O．7％-1．3％的纤维素酶、0．6％-1．0％果胶酶和10％甘露醇组成，pH5．8，酶解时间20-30min。分离的蝴蝶兰早期原胚和接近成熟的球形原胚均发现有发达的胚柄吸器。
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| 关 键 词： | 蝴蝶兰 合子 原胚 胚柄吸器 分离操作 |
Isolation of Zygotes and Embryos in Phalaenopsis |
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| Wu Chenghou,Li Dongmei,Tian Huiqiao.Isolation of Zygotes and Embryos in Phalaenopsis[J].Chinese Journal of Cell Biology,2012(4):361-365. |
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| Authors: | Wu Chenghou Li Dongmei Tian Huiqiao |
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| Institution: | (J School of Life Sciences, Xiamen University, Xiamen 361005, China; 2Guangzhou Institute of Landscape Gardening, Guangzhou 510405, China; 3Shunde Polytechinic, Shunde 528300, China) |
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| Abstract: | Zygotes, proembryos and maturing spherical proembryos were isolated from Phalaenopsis hybrid embryo sacs by the method of manual microdissection after a pretreatment of combinnating enzymatic mac- eration with shaking the ovules. The enzymatic maceration was under 0.7%-1.3% cellulase, 0.6%-1.0% pectinase, 10% mannitol, pH5.8 for about 20-30 min. Developed suspensor haustorium was discovered on the micro-dissect- ed embryos from three-cell stage to maturing spherical. |
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| Keywords: | Phalaenopsis zygote proembryo suspensor haustorium isolation |
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