Isolation of a cDNA encoding a CHH-family peptide from the silkworm Bombyx mori |
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Authors: | Endo H Nagasawa H Watanabe T |
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Affiliation: | Laboratory of Molecular Biology of Marine Organisms, Ocean Research Institute, The University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo, Japan. |
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Abstract: | The crustacean hyperglycemic hormone (CHH) peptide family includes four types of neuropeptide in decapod and isopod crustaceans, and the ion-transport peptide in orthopteran insects. To identify a new member of this family in Insecta, a PCR-based search for cDNAs encoding CHH-family peptides was carried out in the silkworm Bombyx mori. A cDNA, named BmCHHL (Bombyx mori CHH-like protein), with an open reading frame of 110 amino acids was isolated. Sequence analyses suggested that the conceptual protein was a precursor of a peptide of 72 amino acids which was amidated at the carboxy terminus. The BmCHHL sequence exhibited significant similarities to members of the CHH family including the orthopteran ion-transport peptide. BmCHHL expression was detected in five or six cells (per hemisphere) in the frontal area of the brain in day 4 fifth instar larvae. |
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