Platelet-derived growth factor elicits cyclic AMP accumulation in Swiss 3T3 cells: role of prostaglandin production |
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Authors: | E Rozengurt P Stroobant M D Waterfield T F Deuel M Keehan |
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Institution: | 1. Imperial Cancer Research Fund P. O. Box 123 Lincoln''s Inn Fields London WC2A 3PX UK;1. Departments of Medicine and Biological Chemistry Washington University School of Medicine The Jewish Hospital of St. Louis 216 South Kingshighway St. Louis, Missouri 63110 USA |
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Abstract: | Partially purified porcine PDGF or purified human PDGF in the presence of phosphodiesterase inhibitors caused marked accumulation of cAMP in Swiss 3T3 cells. The responses were time- and dose-dependent; half-maximal effect was obtained at 0.6 nM PDGF. Indomethacin prevented the increase of cAMP levels in a dose-dependent manner; half-maximal effect was obtained at about 10 nM. Addition of PDGF increased (at least 25-fold) the production of E-type prostaglandins; PGE reached a concentration in the medium of 26 ng/ml 1 hr after treatment with human PDGF. This concentration of PGE produced a similar level of cAMP to that found with PDGF, suggesting that the PDGF-induced increase in cAMP is mediated by E-type prostaglandins released in the culture medium. Increased cAMP levels promoted by PDGF acting through stimulation of E-type prostaglandin synthesis may contribute to signal the initiation of cell proliferation in 3T3 cells. |
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