Coding Sequence, Genomic Organization, Chromosomal Localization, and Expression Pattern of the Signalosome ComponentCops2:The Mouse Homologue ofDrosophila Alien |
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| Authors: | Laura Schaefer Mary Lou Beermann Jeffrey B Miller |
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| Institution: | a Myogenesis Research Laboratory, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts, 02129;b Program in Neuroscience, Harvard Medical School, Boston, Massachusetts, 02115 |
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| Abstract: | TheDrosophila aliengene is highly homologous to the human thyroid receptor interacting protein, TRIP15/COPS2, which is a component of the recently identified signalosome protein complex. We identified the mouse homologue ofDrosophila alienthrough homology searches of the EST database. We found that the mouse cDNA encodes a predicted 443-amino-acid protein, which migrates at 50 kDa. The gene for the mousealienhomologue, namedCops2,includes 12 coding exons spanning 30 kb of genomic DNA on the central portion of mouse chromosome 2. MouseCops2is widely expressed in embryonic, fetal, and adult tissues beginning as early as E7.5. MouseCops2cDNA hybridizes to two mRNA bands in all tissues at 2.3 and 4 kb, with an additional 1.9-kb band in liver. Immunostaining of native and epitope tagged proteins localized the mouseCops2protein in both the cytoplasm and the nucleus, with larger amounts in the nucleus in some cells. |
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