Relationship between expression of the PM H+-ATPase,growth and ion partitioning in the leaves of salt-treated Medicago species |
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Authors: | John?V.?Sibole author-information" > author-information__contact u-icon-before" > mailto:dbsjsi@uib.es" title=" dbsjsi@uib.es" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Catalina?Cabot,Wolfgang?Michalke,Charlotte?Poschenrieder,Juan?Barceló |
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Affiliation: | (1) Departament de Biologia, Universitat de les Illes Balears, Cra. Valldemossa km 7.5., 07122 Palma de Mallorca, Spain;(2) Institut für Biologie III, Universität Freiburg, Germany;(3) Laboratori de Fisiologia Vegetal, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain |
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Abstract: | The role of the plasma membrane (PM) H+-ATPase (E.C. 3.6.1.3) in the plants response to salt stress was studied in the perennial leguminosae forage Medicago arborea L. and its close relative Medicago citrina (Font-Quer) Greuter, a species exposed to saline conditions in its original habitat. Plants were solution cultured for 8 days in 1 or 100 mM NaCl. Leaf growth and CO2 assimilation were more inhibited by salt in M. arborea than in M. citrina. Both species were able to osmoregulate, and salt-treated plants maintained turgor potentials, with no differences between species. Contrasting ion distribution patterns showed that M. citrina was able to exclude Na+ from the leaves more selectively, while M. arborea had a greater buildup of leaf blade Na+. Isolation of purified PM and quantification of H+-ATPase protein by Western blot analysis against the 46E5B11D5 or AHA3 antibodies showed an increase in response to salt stress in the expanding (92%) and expanded leaves (87%) of M. citrina, while no differences were found in the corresponding leaves of M. arborea. The assay of H+-ATPase specific activity of the two leaf types in salinized M. citrina confirmed this increase, as activities increased with 55% and 104% for the expanded and expanding leaves, respectively, while no significant differences were found for either leaf type of salinized M. arborea. A possible role of the increased expression of the PM H+-ATPase for leaf expansion and ion exclusion in salt-stressed plants is discussed. |
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Keywords: | Salinity (salt stress) H+-ATPase (plasma membrane) Medicago Plasma membrane |
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