Fts insertional mutant of Salmonella typhimurium |
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| Authors: | M. Cristina Cerquetti Rolando Brawer Christian A. Gerdes M. Magdalena Gherardi Daniel O. Sordelli |
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| Affiliation: | US Department of Agriculture, Forest Service, Forest Products Laboratory, 1 Gifford Pinchot Drive, Madison, WI 53705-2398, USA |
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| Abstract: | Abstract Cellulolytic and xylanolytic polysaccharidase enzyme activities from the multi-enzyme complex of Postia placenta MAD 698 were dissociated. Decayed wood extracts were fractionated by methyl-hydrophobic interaction chromatography and analyzed for reducing sugars and by viscosimetry and Western blot. Fractionated endoglucanases had molecular masses of 35 and 40 kDa on SDS-PAGE. Western blots probed with anti-endoglucanase and anti-xylanase antibodies revealed two endoglucanase bands at 35 and 40 kDa, but no commonality with the major xylanase band at 36 kDa. Anti-endoglucanase antibody inhibited endoglucanase enzyme activity. |
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| Keywords: | β-1,4-Endoglucanase Carboxymethylcellulase Postia placenta Brown-rot Hydrophobic interaction chromatography |
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