| 不同工艺提取老鹰茶总黄酮含量的比较 |
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| 引用本文： | 陆维丽,秦敬克,汤文建,李俊. 不同工艺提取老鹰茶总黄酮含量的比较[J]. 生物学杂志, 2009, 26(3): 66-67. DOI: 10.3969/j.issn.1008-9632.2009.03.066 |
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| 作者姓名： | 陆维丽 秦敬克 汤文建 李俊 |
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| 作者单位： | 安徽医科大学药学院,合肥,230032 |
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| 基金项目： | 安徽省科技攻关项目，安徽高等学校省级自然科学研究基金 |
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| 摘 要： | 比较不同工艺提取富集的老鹰茶中总黄酮含量,分别采用传统乙醇提取-正丁醇萃取法和水提取-大孔树脂法提取老鹰茶总黄酮,并用紫外分光光度法,以芦丁为标准品,270nm下测定总黄酮含量。水提取-大孔树脂法富集所得固体中黄酮含量较高,可达55.86%,回收率为105.6%,RSD=1.64%。水提取-大孔树脂富集的工艺操作简单,所得固体黄酮含量高,更适于老鹰茶总黄酮的提取,对进一步的工业化生产开发有较好的参考价值。
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| 关 键 词： | 老鹰茶 总黄酮 提取工艺 紫外分光光度法 |
Comparison of the total flavonoids contents of Litsea coreana leaf in different extraction methods |
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| LU Wei-li,QIN Jing-ke,TANG Wen-jian,LI Jun. Comparison of the total flavonoids contents of Litsea coreana leaf in different extraction methods[J]. Journal of Biology, 2009, 26(3): 66-67. DOI: 10.3969/j.issn.1008-9632.2009.03.066 |
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| Authors: | LU Wei-li QIN Jing-ke TANG Wen-jian LI Jun |
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| Affiliation: | (Anhui Medical University, College of Pharmacy, Hefei 230032, China) |
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| Abstract: | To compare different extraction methods for the total flavonoids of Litsea coreana leaf, the contents of the total fla- vonoids of Litsea coreana leaf are determined by using the methods of traditional ethanol-n-butanol extraction and water-macro- porous resin extraction, and UV spectrophotometer. The content of the total flavonoids in the water-macroporous resin extraction is higher, up to 55.86 % , and the recycling rate is 105.6 % , RSD = 1.64 %. The method of water-macroporous resin extraction is simple, with the high total flavonoids content. This method is more suitable for extracting the total flavonoids of Litsea coreana leaf, and is useful to the further development in industrial production. |
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| Keywords: | Litsea coreana L flavonoids extraction method UV spectroscopy |
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