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影响辣椒离体再生芽伸长因素研究
引用本文:张余洋,汤银珠,叶志彪,张婵娟,李汉霞. 影响辣椒离体再生芽伸长因素研究[J]. 武汉植物学研究, 2007, 25(2): 185-191
作者姓名:张余洋  汤银珠  叶志彪  张婵娟  李汉霞
作者单位:1. 华中农业大学,国家蔬菜改良中心华中分中心,武汉,430070;华中农业大学,作物遗传改良国家重点实验室,武汉,430070
2. 华中农业大学,国家蔬菜改良中心华中分中心,武汉,430070;中国科学院武汉植物园,武汉,430074
3. 华中农业大学,作物遗传改良国家重点实验室,武汉,430070
4. 华中农业大学,国家蔬菜改良中心华中分中心,武汉,430070;华中农业大学,园艺植物生物学教育部重点实验室,武汉,430070
摘    要:以辣椒子叶为外植体,比较不同浓度BA和IAA激素组合对辣椒再生芽诱导的差异,利用筛选出的高效芽诱导培养基为基础,研究了赤霉素、芽诱导时间、培养基有机成分、不同激素组合和品种等因素对辣椒不定芽伸长的影响。结果表明:不同基因型辣椒子叶再生能力不同,BA3.0mg.L-1 IAA0.5mg.L-1的激素配比对不定芽诱导频率最高;不定芽的伸长百分率随着GA3浓度的增加而增加,GA3的适合浓度为1.0~2.0mg.L-1;不定芽诱导时间对不定芽的伸长有一定的影响,诱导21d的不定芽,其伸长频率明显高于诱导14d的不定芽;B5有机成分在辣椒不定芽的伸长中效果优于MS有机成分;激素组合对不定芽伸长有一定的影响,Zeatin GA3激素组合对伸长效果最好,BA IAA GA3伸长效果较好,BA PAA(苯乙酸,phenylaceticacid) GA3伸长效果次之;不同品种辣椒不定芽的伸长能力有一定差异,楚风和苏椒五号再生芽伸长能力最佳。与IAA和NAA相比,IBA对再生芽生根效果较好。

关 键 词:辣椒  子叶  再生  芽伸长
文章编号:1000-470X(2007)02-0185-07
收稿时间:2006-08-14
修稿时间:2006-08-14

Factors Affecting in vitro Shoot Elongation of Pepper
ZHANG Yu-Yang,TANG Yin-Zhu,YE Zhi-Biao,ZHANG Chan-Juan,LI Han-Xia. Factors Affecting in vitro Shoot Elongation of Pepper[J]. Journal of Wuhan Botanical Research, 2007, 25(2): 185-191
Authors:ZHANG Yu-Yang  TANG Yin-Zhu  YE Zhi-Biao  ZHANG Chan-Juan  LI Han-Xia
Affiliation:1. National Center for Vegetable Improvement ( Central China
Abstract:A detailed study was carried out to investigate key factors affecting shoot elongation of pepper. Using the cotyledons of pepper as explants, the optimal phytohormone combination of BA+IAA for bud induction was screened out to investigate the effect of GA3, bud-inducting time, organic constituents in media, different phytohormone combinations and cultivars on shoot elongation. The results were as follows:Seven cultivars showed ability differences for bud induction,and BA 3.0 mg·L-1+IAA 0.5 mg·L-1 could give the highest response. The shoot elongation frequency increased with GA3 concentration, with the optimal concentration of 1.0-2.0 mg·L-1. The buds subjected to 21-day induction showed higher elongation efficiency than that of 14-day induction.B5 vitamins were more efficient than MS vitamins for shoot elongation. The phytohormone combination of Zeatin+GA3 exhibited higher elongation efficiency than combinations of BA+IAA+GA3 or BA+PAA+GA3.Elongation ability varied with pepper cultivars, with Chufeng and Sujiao No.5 having the best elongation ability. Compared with IAA and NAA, IBA gave better rooting effect.
Keywords:Capsicum annuum L.  Cotyledon  Plant regeneration  Shoot elongation
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